Core Technology

Core Technology

The Center manages several technology platforms that are maintained by the NYUAD CTP team. Please visit our sister center in NY to learn about what is offered there:

Sequencing

The Human Genome Project was completed in ten years and cost three billion dollars. Due to recent advances in high-throughput sequencing technology, an entire human genome can now be sequenced in a few days for one millionth of the original cost. The Sequencing CTP is used by researchers in genomics and systems biology to rapidly collect large amounts of data on DNA and RNA sequences from humans, vertebrate and invertebrate animals, plants, and microbes.

Applications include:

  • Analysis of genomes
  • Gene expression patterns
  • Regulatory interactions
  • Epigenetics
  • Sequence Diversity

Bioinformatics Core

The Bioinformatics Core consists of an expert team of analysts dedicated to assisting researchers understand their data. The breadth of knowledge covers all of the Core Technology Platforms offered at NYU and stretches far beyond. Some of the most frequent requests within NYU are:

  • Study Design
  • Analysis Pipeline Implementation
  • Sequence Assembly
  • ChipSeq Analysis
  • Methodological Comparisons

High-Throughput Screening

The High-Throughput Screening CTP is used by biologists and chemists to accelerate their research through laboratory automation, enabling researchers to perform thousands of tests in parallel using biological and chemical materials. This technology is used in drug discovery, toxicity studies, and cell biology research.

Light Microscopy

The Light Microscopy CTP is equipped with instruments that enable imaging of both the surface and depth of live cells and fixed biological samples. Equipment in this CTP can be used to obtain high-resolution images of fluorescent samples at specific depths and can produce scans of moving neurons at up to 428 frames per second.

Fluorescence Activated Cell Sorting (FACS)

The Flow Cytometry Core is equipped with BD FACSAria III which measures and then analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include the particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence

It is widely used in areas of research that require analysis or isolation of cells from suspension. This technology makes use of fluorescent probes targeted to specific cell-associated molecules to characterize the diversity and function of complex cell populations. In addition to analysis,fluorescence-activated cell sorters can individually identify and isolate live cells with a defined phenotype that can later be expanded and/or further studied.

Common applications of flow cytometry include the analysis and/or sorting of experiments studying:

  • Apoptosis (intracellular homeostasis, free radical production, chromatin condensation, DNA fragmentation, plasma membrane integrity, mitochondrial function, caspase activation, phosphatidylserine translocation, etc.)
  • Cell viability
  • Cellular signal transduction (calcium flux, cytokine studies, intracellular pH and glutathione measurements, cell cycle analysis, cellular transport assays, drug uptake/efflux assays
  • Characterization of multi-drug resistance (MDR) of cancer cells
  • DNA (cell cycle analysis, cell kinetics, proliferation, etc.)
  • RNA
  • Immunophenotyping (cell surface antigens, intracellular antigens, etc.)
  • Transfection efficiencies

And many more!